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FBA was performed using ATP hydrolysis as the objective function, formulated to maximize ATP production potential under steady-state constraints

Two ml basic medium containing 40 g/ml DNAse I, 0.25 mg/ml SBTI, and 3 mM MgSO 4 in HBSS was added to the brain cubes and mixed gently up and down with a pipette without making foams for 30 times

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At a healthy state, the dynamic equilibrium of iron is well maintained and the iron metabolism is regulated by a variety of factors, including hepcidin (47, 48), HIF-2 (51), Nrf2 (52), and the gut microbiota (46)

Chirumbolo, Thymosin 4: A Multi-Faceted Tissue Repair Stimulating Protein in Heart Injury