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The analytical conditions were as follows: both the run and rinse buffers were anion buffer solutions (p/n: H3302-1023), sample injection was performed by pressure injection at 50 mbar for 25 s, the CE voltage was maintained at 30 kV, and MS ionization was performed in both positive and negative ESI modes

GSIS values were calculated by subtracting the insulin concentrations measured after incubation in the low-glucose buffer from those measured in the high-glucose buffer

reported that during rapid freezing, small, iced crystals can form that can minimise disruption of the bilayer structure

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