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Notably, Ataluren forms hydrogen bonds with His 153 and Arg 174 , a salt bridge with Arg 174 , and a -stacking interaction with Phe 113

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After centrifugation, 800 L 2.5 mol L 1 H 2 SO 4 was used to fully dissolve the precipitate, followed by centrifugation at 8,000 g for 5 min at 20 C

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