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The islets were further incubated in 50 L of KRH solution with 16.7 mM glucose (high-glucose buffer), supplemented with 2 M of unfused WT albumin, GLP-1 WT, GLP-1 QMP, or Exendin-4 (Byetta) (positive control)

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We found that expression of GPX4 was reduced after Cys 2 starvation and erastin treatment, further proving that ferroptosis is a primary mechanism of GSH depletion-induced cell death in RPE (Fig

237 In addition, trichostatin A, an HDAC inhibitor, blocks upregulation of uremia environment-induced markers of endothelial dysfunction (intercellular adhesion molecule-1, surface Toll-like receptor-4, von Wilebrand factor) and reactive oxygen species