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Accumulated ROS cause oxidative damage to lipids, DNA, and proteins, creating a pro-degenerative cellular environment (Chang and Chen, 2020

The limiting dilution sphere formation assay showed that the cells that were treated 20 days earlier with the combination of sertraline and 6 Gy irradiation were intrinsically rewired and presented a decreased sphere formation efficiency, indicating a 3-fold reduction and loss of long-term self-renewal capacity ( p = 0.0061) (Fig