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You may begin to notice results within 24 to 48 hours for moderate to severe frown lines* *Source: BOTOX Cosmetic Site BEFORE TREATMENT Avoid aspirin (also Excedrin), Vitamin E, Saint John's Wort and other dietary supplements including ginkgo, evening primrose oil, garlic, feverfew, and ginseng for 2 weeks Avoid ibuprofen (e.g