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The cDNA product was diluted and processed for real-time PCR quantification using the QuantStudio 5 real-time PCR system (Thermo Fisher Scientific, Waltham, MA)

The Connectivity Map data comes from cell lines treated in vitro

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8 weeks (1 vial): 12 mL 1 10 mL bottle 12 weeks (2 vials): 24 mL 1 10 mL bottle 16 weeks (3 vials): 36 mL 1 10 mL bottle Alcohol Swabs One for the vial stopper + one for the injection site each week