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Finally, the cells were incubated with calcein acetoxymethyl ester (calcein AM) and propidium iodide (PI) at 37 C for 30 min

This groundbreaking discovery is credited to the Stockwell Laboratory at Columbia University in 2012, marking a significant advancement in the field [10]

In Bacterial Lipopolysaccharides: Structure, Chemical Synthesis, Biogenesis and Interaction with Host Cells (eds Yuriy A

Das, S.S., Gopal, P.M., Thomas, J.V., Mohan, M.C., Thomas, S.C., Maliakel, B.P., Krishnakumar, I.M., Sasidharan, B.C.P.: Influence of CurQfen-curcumin on cognitive impairment: a randomized, double-blinded, placebo-controlled, 3-arm, 3-sequence comparative study

Finally, to provide further proof that HuR controls KGA levels, we used the CRISPR-Cas9 knock-in system to modify the GLS gene by introducing a fluorescence protein (mKO2 49 ) within exon 19 to express KGA fused to mKO2 at its C-terminus (Fig