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Lodise, T., Corey, R., Hooper, D

doi:10.1016/j.bbamcr.2016.01.023

Overexpression of AKR1C3 significantly enhances human prostate cancer cells resistance to radiation

Affinity studies of DHA for GLUT1 may help establish a lower effective dose, though unwanted side effects in tissues that highly express GLUT1 need to be considered

In order to recover GST-tagged protein from the complicated mixture of proteins, the fusion protein is incubated with agarose beads coupled to glutathione (GSH-agarose, see picture below), what leads to efficient precipitation of GST-fusion protein