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However, in line with the specificity of CPA, the C-terminal residues of the L585I, L585M, and L585V variants were cleaved in vitro upon exposure to the enzyme for 5 hours at 37 C, as confirmed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis of the C-terminal peptides (Supplementary Table 1)

But, on the other hand, betaine supplements may also raise cholesterol levels in some people.39 Also, its worth noting that the dose of betaine in Codeages supplement (102.5mg) is very low compared to those used in studies