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The membrane was blocked with 5% skimmed milk in 100 mM Tris-HCl (pH 7.5), 150 mM NaCl and 0.2% Tween-20 (TBST) for 1 h at room temperature

3.2 NTHi acquisition of host-derived haem-iron sources Despite the inherently low availability of haem-iron in the respiratory tract, NTHi is adept at scavenging a variety of host-derived haem-iron sources from intracellular and extracellular reservoirs, using a highly complex and redundant repertoire of haem acquisition systems ( Table 1 )

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For example, in trauma to the brain or spinal cord, the injurious free radicals are in the fatty (lipid) coverings that insulate nerve fibers, the myelin sheaths

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