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doi: 10.1002/alz.13844, 214

Although evidence has shown the presence of XO in the human brain, which catalyzes purine metabolism to UA, the concentration of UA generated in situ in the brain is low

The slides were washed in water and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

Another explanation could be that these adducts may break into lactone and the substrates (for example, secondary amines) of former Micheal-type addition reaction, due to the reversibility of this type of reaction (Lawrence et al., 2001)

This is made even more problematic, given the number of factors that can deplete glutathione levels