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Quantification of RUNX and BMP-2 RUNX (MBS2509507, MyBioSource, San Diego, USA) and BMP-2 (MBS701336, MyBioSource, San Diego, USA) levels were detected in aorta using rat specific enzyme-linked immunosorbent assay (ELISA) kits following the manufactures instruction

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The same applies to micronutrients

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