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While there are different models to study glutathione metabolism, in this study we used BSO as it is a specific and potent chemical inhibitor of the rate limiting enzyme GCLC (38)

Drug Design, Development and Therapy , 18, 2971 2987

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Screening by determining total homocysteine (tHcy) in dried blood spots is only occasionally performed given the need for a reduction step prior to LC-MS/MS assay and associated costs [42]