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Briefly, 100 l of fresh mitochondria extracted from liver tissues were mixed with 900 l staining working solution and then the fluorescence intensity was assessed using a fluorescence microplate reader (BioTek, Winooski, VT, USA)

Impaired cardiac functional reserve in type 2 diabetic db/db mice is associated with metabolic, but not structural, remodelling

It should be noted that the activation of the glutamate-nitric oxide-cGMP pathway modulates different cerebral processes, such as the sleep-wake cycle, long-term potentiation (LTP), and some types of learning and memory (Sistiaga et al., 1997)