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RT-qPCR analysis was performed on a QuantStudio 3 Real-Time PCR Detection System using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02) with specific primers

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This is called mineral/vitamin toxicity or toxemia

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For instance, in a randomized, placebo-controlled trial in healthy adults, subcutaneous CJC-1295 injections produced sustained, dose-dependent increases in GH (two to 10 times) and IGF-1 (1.5 to three times) levels over several days, demonstrating prolonged GH axis stimulation without serious adverse effects, a hormonal milieu associated with anabolic and metabolic effects