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We receive our first gut microbes as babies and our diet and environment adds new ones after that - though environmental exposure can also sometimes harm the gut and reduce the number of microbes

The formalin-fixed liver specimens were inserted in liquified paraffin wax and cut into 5-m thick sections utilizing a Leica microtome (Leica RM 2155, England)

For the T cell adoptive transfer experiments, MC38-ASbearing mice were sublethally irradiated with 600 cGy on day 7