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membrane processing microfiltration (MF) and/or ultrafiltration (UF) membranes may be used for concentrating whey

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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

The pivotal role of sex steroids on the setpoint of the axis is reflected by the peak circulating levels of IGF-I and IGFBP-3 reached in mid-puberty (50,51)

However, the regulatory mechanism remains unknown