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Mechanistically, these protective effects can be attributed to diminished SMC necroptosis which leads to reduction in the release of proinflammatory cytokines and DAMPs [11] via rapid permeabilization of the cell membranes as well as preventing release of inflammatory mediators activating pattern recognition receptors (PPRs) on neutrophils and macrophages [39]

To observe the fixed cell with the taken-up EVs, 1 10 5 of PANC-1 cells stably expressing mEos4b-Lamp1 were seeded on glass-based dishes 2 days before EV addition