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cerevisiae ubiquitin ( sc Ub) and the I3C mutant were cloned into the pK27(His14-SUMO- hs Ub) vector using the Gibson assembly method

The H values obtained in this work are lower than the 11.4 J/g that was reported for buckwheat globulin (Tang & Wang, 2010) and 75.93 J/g for chickpea protein isolate (Ghribi et al., 2015) but some of them are slightly higher than the 0.16 J/g for lentil protein isolates (Miranda et al., 2022)