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The Agilent 1200 series system (Agilent Technologies, Waldbronn, Germany) was used with a reversed phase precolumn (LiChrospher 100 RP18 EC-5, 40 4 mm) (CS-Chromatographie, Langerwehe, Germany) and a reversed phase main column (LiChrospher 100 RP18 EC-5, 125 4 mm) (CS-Chromatographie, Langerwehe, Germany) and methanol:water (9:1) (A) and methanol (B) were used as mobile phases

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10.1093/hmg/ddx410 Hum

Verify the current MAC LCD for the most recent coverage determination before billing semaglutide or tirzepatide for non-diabetes indications Source: CMS LCD Database RCM teams managing injection billing must verify that their billing software reflects the April 2026 quarterly MPFSDB update