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Description

85011433) were purchased from the European Collection of Authenticated Cell Cultures (ECACC) and were cultured in DMEM (Sigma-Aldrich) supplemented with 10% FBS, 1 mM HEPES, 100 mM sodium pyruvate, and 1x Tryptose Phosphate Broth (Gibco)

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The European Food Safety Authority (EFSA) sets the adequate intake (AI) for adolescents and adults at 4 g vitamin B12 per day

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