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Experimental studies show that nighttime administration of melatonin increases the expression of occludin, claudin-5, and ZO-1, stabilizes the cytoskeleton, and reduces BBB leakage after ischemia

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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3 UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3 UTR reporter plasmid (mut)