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Sequence alignment of the C-terminal HF domains from 35 Taf12 homologs across multiple species including Candida, Saccharomyces, Schizosaccharomyces pombe, Arabidopsis thaliana, Drosophila melanogaster, Mus musculus , and Homo sapiens revealed strong conservation across the three core -helices (1, 2, 3) and the C-terminal C helix (Figure S5B)

For butyrylation, butyrate is activated by ACSSs to generate butyryl-CoA, which is then utilized by acyltransferases like p300/CBP and Gcn5 to modify target proteins

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Added stress can make a gout attack feel more intense