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For each sample, three replicates were performed and relative expression ratio was calculated using delta Ct value method

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Furthermore, recent studies indicate that folic acid therapy in a daily dosage of 0.5 mg is the lowest effective therapy for reducing both fasting and postmethionine-load homocysteine concentrations in hyperhomocysteinemic subjects, with similar effects in a daily dosage of 5 mg (Clarke and Armitage, 2000

Alpha-tocopherol may influence cellular signaling by modulating jasmonic acid levels in plants