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Colorimetric metal assay The holo-SOD1 WT (50 M, monomer based) was incubated for 20 h at 37 C in 50 mM Hepes buffer, 50 mM NaCl, pH 7.4, in the presence of the indicated concentrations of thiol compounds and 100 M divalent metal dye 4-(2-pyridylazo)resorcinol (PAR), in 90 l total volume in 384-well plates covered with a transparent adhesive film (to prevent evaporation)

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