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After staining, sections undergo a series of alcohol dehydrations, clearing in xylene, and are then mounted with a neutral resin before being examined under a microscope (BDS400-FL, Ote, Chongqing) for histological evaluation

In regards to the temperature stability of hyaluronidase, a 2015 patent highlights that the uber-thermophile modified variant retains at least 50 % of its enzymatic activity after incubation at 52 C (125.6 F) for 10 min at a neutral pH (Halozyme Inc., 2015), whereas the unmodified variant exhibits significantly reduced activity under similar conditions (Chen et al., 2016)

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