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The precipitated proteins were washed twice with 500 L methanol and resuspended with 800 l of PBS containing 0.5% SDS, and then labeled with 5 mM DiaAlk probe at 37 C for 2 h with rotation and light protection

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Neuronal resilience to energy-demanding states is therefore limited and depends on energy substrates supplied by neuroglia, particularly astrocytes 587,588,589,590

In diabetes, somatostatin levels are increased in pancreas and gut, presumably as a consequence of insulin deficiency

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