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Avoid layering directly with high-dose Vitamin C or strong acids in the same step, as these can destabilize the copper complex

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Conversely, the authors also found that copper deficiency decreases the formation of autophagy-initiating phagophores, which contain important ULK1/2 downstream targets ( Figure 1 )

Can also be utilized in the modulation of transcriptional regulators like EGR1 and NAB2, which play a role in gene expression during stress responses (3)