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In animal models, intranasal administration of GHK-Cu improved cognitive functions, reduced amyloid plaques, and decreased inflammation in the brain, suggesting its potential as a therapeutic agent for Alzheimers disease and age-related cognitive decline 10

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2.5 Determination of metabolite levels and oxygen consumption in isolated mitochondria To determine levels of KC metabolites, mitochondria (10 mg protein/mL) were incubated at 37C in KME buffer plus 5 mM Pi, 0.2 mM ADP and one of the following substrates: 2 mM Pyr plus 5 mM Mal, 4 mM glutamine, 10 mM 2-oxo or 5 mM Suc plus 2 M rotenone for 10 min under orbital shaking at 150 rpm

Using cell-to-cell variabilityA new era in molecular biology