multidrug resistance protein 1 glutathione ABCC1 Identification of human multidrug resistance

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Nonetheless, the increased demand for energy and building blocks for macromolecule synthesis upon mTORC1 activation, together with the block in autophagy, results in a biosynthetic burden that the cell alleviates with the execution of parallel programs that reinforce nutrient uptake (Torrence et al., 2020) and synthesis (Valvezan et al., 2017), and also enables the catabolic capacity of the cells through the proteasomal degradation of proteins (Zhang et al., 2014) to remove unwanted cellular material and to boost the pool of free amino acids available for protein synthesis

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Blood was collected in EDTA tubes and centrifuged at 1,600 g for 16 min at room temperature to separate the plasma, which was aspirated and stored at 80 C