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This study concluded that the high doses of Fe 3 O 4 @Ag-NPs treatment can be toxic and can exhibit harmful effects on living organisms [192]

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Response of mouse zygotes treated with mild hydrogen peroxide as a model to reveal novel mechanisms of oxidative stress-induced injury in early embryos

Most handling guides recommend gentle mixing (swirl/roll) rather than vigorous shaking to reduce foaming, bubbles, and airliquid interface effects that can increase losses

Toxicity and Data Limitations: No chronic toxicity data exist for this blend