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Glutathione biosynthesis occurs in two sequential, ATP-dependent enzymatic steps that take place in the cytosol: Step 1: The enzyme glutamate-cysteine ligase (GCL) combines glutamate and cysteine to form gamma-glutamylcysteine

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doi: 10.1016/j.bbadis.2020.166037 194

Concurrently, metabolic reprogramming, particularly the shift toward glycolysis, enhanced mitochondrial biogenesis and lipid metabolism, enables the bioenergetic demands of clonal expansion and effector differentiation 9