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In brief, the proteins were precipitated with 0.5 mL ice cold 10% 5-sulfosalicylic acid and the tubes were incubated on ice for 10 min, centrifuged (Sorvall Instruments RC5C, DuPont, Minnesota, USA) for 15 min at 15,000 rpm at 4C

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For reconstitution itself, a larger syringe like a 3mL or 5mL syringe is often easiest for accurately measuring the volume of BAC water