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Risk analysis of colorectal cancer incidence by gene expression analysis

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Cells were cultured in 200 L Neurobrew (Milteny Biotec, Bergisch Gladbach, Germany), 100 L N2-supplement (Thermo Fisher Scientific, Waltham, MA), 2 L bFGF (PeproTech, Princeton, NJ) and 2 L PDGF-BB (R&D Systems, Minneapolis, MA) per 10 mL of DMEM/F-12 media (Thermo Fisher Scientific, Waltham, MA)