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These small differences in efficacy of BLX2477 may be explained by the 3-log higher IC 50 of BLX2477 for free versus PE-esterified eicosatetraenoic acid (ETE) (Supplemental Figure 3)

Two-group comparisons of matched data sets were performed using the Wilcoxon test, as the number of experiments did not allow testing for normality distribution

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Previous efforts to map polyubiquitin-binding sites on Ufd1 using the hydrogen-deuterium exchange coupled with mass spectrometry technique (HDX/MS) found that both the ridge and cleft sites showed reduced HDX rate upon polyubiquitin binding ( Extended Data Fig