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J Hematol Oncol 15 , 65 (2022)

At the molecular level, it seems that the mechanisms of action vary depending on the dose of metformin used and duration of treatment

The protein concentrations in the supernatants were determined by Lowry's method [18]

PMID 29767158

Brain lipid extraction and preparation of large unilamellar vesicles (LUVs) LM or PM-enriched fractions obtained from WT newborn (P1) mice (approximately 100 mg protein in NB buffer) were mixed with 25 ml of a 2:1 chloroform:methanol solution at room temperature