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doi: 10.3390/antiox10030351 131 MoschouP

In a clinical review, serum melatonin levels were reported to be significantly lower and the time of peak melatonin values delayed in elderly subjects with insomnia compared with age-matched healthy controls

Belanger CF, Hennekens CH, Rosner B, et al

10.1093/abbs/gmr079 Acta Biochim

For OCT embedding, fixed tissues were cryoprotected in 15% sucrose (Sigma-Aldrich, S9378) at 4 C for 24 h, followed by 30% sucrose at 4 C for an additional 24 h