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All OPERA predictions are provided with AD and accuracy estimates as well as experimental and predicted values for the nearest neighboring chemicals as shown on the EPA Dashboard prediction reports and explained in Mansouri et al

Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 l mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added

We averaged the prediction errors for duplicate molecules across multiple runs and classified each pKa site into one of the 18 functional groups we identified based on the GNNs internal sub-graph logic