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Poropat, G., Giljaca, V., Stimac, D

CYP2C19 variation and citalopram response

The lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten minutes at 95 C