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Wolfram Brune, Leibniz Institute of Virology) cells were cultured at 37 C and 5% CO 2 in Dulbeccos modified Eagles medium with glutamine (DMEM) (Gibco, 41966-052) supplemented with 10% (v/v) fetal bovine serum (FBS) (Sigma-Aldrich, F7524) and 1% (v/v) non-essential amino acids (Gibco, 11140-035)

Expression and distribution of insulin-like growth factor-1 receptor in human carcinomas

Cells were then incubated in ChIP lysis buffer (50 mM Tris-HCl pH 8.0, 5 mM EDTA, 150 mM NaCl, 1% NP-40, 0.1% SDS, with protease inhibitor) for 30 min on ice

Lipoxygenase Lipoxygenases (LOs) are a family of enzymes that catalyze the addition of oxygen to polyunsaturated fatty acids (PUFAs), specifically those containing a 1,4-pentadiene structure [245]