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At the thigh in lean patients, 45 degrees with a pinch is safer to avoid muscle penetration

Diverse Biological Activities The peptide's biological activities are remarkably diverse and include stimulation of collagen and glycosaminoglycan synthesis (critical for skin, connective tissue, and wound healing), activation of wound healing mechanisms, promotion of blood vessel formation (angiogenesis) to support tissue repair and regeneration, anti-inflammatory effects that resolve excessive inflammation while supporting beneficial healing responses, antioxidant and protective effects against oxidative damage and toxic compounds, attraction of immune and repair cells to sites of injury, remodeling of extracellular matrix through regulated metalloproteinase activity, stimulation of nerve outgrowth and regeneration, and even modulation of gene expression in patterns that reverse age-related changes and restore more youthful cellular function

Research and clinical experience suggest that IV NAD+ produces a sharp but short-lived spike in plasma levels that does not reliably translate to intracellular increases, which is where the therapeutic effect actually happens