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After incubation for 15 min at room temperature, both solutions were combined and added dropwise to the cells combined

Shi L, Duan R, Sun Z, Jia Q, Wu W, Wang F, et al

Callus proliferation was good at lower concentrations (2 and 4 mg L -1 ), whereas higher concentrations (8 and 16 mg L -1 ) adversely affected the callus formation, resulting in moderate proliferation (Table 3)

Subsequently, fungal cells were stained with Calcofluor White (Sigma-Aldrich) at a final concentration of 1 g/ml and Sytox Orange (ThermoFisher Scientific, Waltham, MA, United States) at a final concentration of 1 M for 10 min in the dark

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