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Western blot analysis Western blotting was performed as previously described via the following antibodies: FBXO22, BACH1, KLF4, LKB1, SNAL1, PTEN, KDM4A, BAG3, and GAPDH (all from Proteintech, Rosemout, IL)

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More than half of women in this life stage suffer from aches, stiffness, and swelling, and symptoms are usually worse in the morning when joints are particularly stiff