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Notably, it is difficult to calculate an accurate FDR using the entrapment strategy given that it is unlikely to know the exact number of phosphopeptides in the human sub-library that is not present in the sample

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The clinic answer In a UK clinic, cold-chain storage, stock rotation and expiry checks are routine, so you never have to think about any of this

If it does not dissolve after 5 minutes of gentle swirling, possible causes include: degraded peptide (check storage history and expiration status ), contaminated water (use a fresh vial of bacteriostatic water), or water temperature (cold water from the refrigerator dissolves peptides more slowly than room temperature water)