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On top of understanding the microbial pathogenesis and the molecular and cellular mechanisms of disease biology, activity of a therapeutic agent in a translational research, be it either in vitro or in vivo, is critical to proffer advancement to first-in-human clinical trials based on laboratory findings of pharmacology, toxicology, and immunology

While the regulatory mechanisms of DNA methylation on LPS remain poorly understood, extensive immunological studies have documented the impact of LPS on DNA methylation patterns in eukaryotic systems 5,169,170,171

In contrast, clinical metabolomic analyses of KD have reported increases in specific BCAAs, such as valine, and improvement of AD-related risk factors such as increased HDL-C and reduced BMI, suggesting that BCAA dynamics may differ depending on the disease stage and metabolic context [35]

Mez J, Daneshyar DH, Kiernan PT, Abdolmohammadi B, Alarez VE, et al

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