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The bound endogenous GS was eluted from cross-linked IgG using Elution buffer pH 2.8 as per the manufacturers protocol

Bae, J.-W

GPX5 is an unusual member of the mammalian GPX family because, unlike the well-known mammalian GPX1-4, it does not contain selenosylcysteine (Secys) at its active site, but instead has cysteine residues (Cys) as its active site (Taylor et al., 2013)

Animal experiments After one week of acclimatisation, 32 rats were randomly divided into four groups ( n = 8/group) as follows: Group 1 (normal control) received saline (0.5 mL/day, P.O.) and a standard diet