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Following normalization using the housekeeping gene, the 2Ct technique was used to calculate the fold changes in the expression levels, as described in 2.7 Detection of aflatoxin residues in eggs The egg was collected after the completion of the trial to detect the residual AFT using high-performance liquid chromatography (HPLC)

Glutathione is one of the bodys most important endogenous antioxidant compounds and serves as a cofactor in natural detoxification processes

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The TGR sample (1/50 volume cell culture) was applied to a 2.5 ml column, which was then washed with 50 ml of TE after which TGR was eluted in ten 1 ml fractions of 1 mM NADPH in TE