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The Michaelis-Menten and Lineweaver-Burk double-reciprocal curves were gained by the steady-state kinetic method (Figures 3C, D)

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The results were also confirmed by Western blot analysis, which showed a higher level of GSTA1 and lower levels of DGAT2 and PLIN2 (Figure 4G), two markers of LD accumulation [18,21], agreeing with our results in cells (Figure 3A,B)

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One hundred seventeen adverse events occurred in 464 patients (25.2%) treated with at least one dose of N -acetylcysteine, and 98 adverse events occurred in 464 patients (21.1%) treated with at least one dose of placebo ( P = 0.16)