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Zpsob uvn glutathionu zvis na jeho form, a u je to orln, injekn, nebo pes ki, a tak na vaem cli

doi: 10.1016/j.pcl.2015.08.008 25 BernasconiSLevyCCohenRGiardinoIPettoello-MantovaniMSomekhE

[DOI] [PubMed] [Google Scholar] 46.Crespo J.M., Boiardi J.L., Luna M.F

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Immunofluorescence and fluorescence complementation For immunofluorescence of cultured cells, the cells were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature, permeabilized with 0.1% TritonX-100 diluted in PBS at room temperature for 5 min, blocked with 10% FBS diluted with PBS for 1 h and primary antibody incubation for 1 h